Dengue Virus, Molecular Detection, PCR, Spinal Fluid
Use
This test aids in the diagnosis of central nervous system infection caused by dengue virus by detecting dengue virus nucleic acid in cerebrospinal fluid (CSF). The presence of dengue virus nucleic acid in CSF or serum serves as a marker for acute-phase infection. It is especially useful within the first week following symptom onset as patients with symptoms persisting for more than a week may test negative and require serologic testing.
Special Instructions
Specimens should be collected within the first week following onset of symptoms for accurate molecular detection. It is important not to centrifuge or heat-inactivate the specimen. Use a sterile screw cap vial for submission. A Microbiology Test Request form (T244) should be sent with the specimen if not ordering electronically.
Limitations
The test may not detect dengue virus if the CSF specimen is collected immediately following infection (<24-48 hours) or if collected more than 7 days after symptom onset. The molecular test might not detect the virus in these periods due to low viral load, and serological tests may be required. Accurate results also depend on proper specimen handling without centrifugation or heat inactivation.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 77958-7
- 77958-7
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile screw cap vial
Collection Instructions
Do not centrifuge or heat inactivate. Preferred collection in vial number 2, though any vial number is acceptable.
Causes for Rejection
Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |