Dengue Virus NS1 Antigen, Serum
Use
This test aids in the diagnosis of dengue virus infection by detecting the dengue virus nonstructural protein 1 (NS1) antigen. The presence of NS1 antigen is suggestive of recent exposure and/or acute infection with dengue virus. It serves as an acute phase marker for infection, being detectable within 24 hours and up to 9 days following the onset of symptoms. Concurrently evaluating NS1 antigen alongside testing for IgM and IgG antibodies to dengue virus provides optimal diagnostic potential for early and late dengue disease phases.
Special Instructions
Though uncommon, false-positive NS1 results may occur in individuals with active infection due to other flaviviruses. In cases where NS1 antigen is negative and infection is still suspected, serologic testing for IgM and IgG antibodies to dengue virus is recommended.
Limitations
The NS1 antigen test may produce negative results if the specimen is collected too soon after infection (<24-48 hours) or more than 9 to 10 days after symptom onset. It should not be used as the sole diagnostic tool; results should be interpreted in conjunction with clinical presentation and patient history. Other flaviviruses may cause cross-reactivity, producing false-positive results.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 75377-2
- 75377-2
- 69048-7
Result Turnaround Time
1-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.4 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into plastic vial. Preferred collection container is serum gel; acceptable is red top.
Storage Instructions
Refrigerated (preferred), 14 days; Frozen, 14 days
Causes for Rejection
Gross hemolysis, Gross lipemia, Gross icterus
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 14 days |