Endomysial Antibodies, IgA, Serum
Use
The analysis of IgA-endomysial antibodies is crucial for diagnosing dermatitis herpetiformis and celiac disease. This test aids in the diagnosis by detecting circulating IgA antibodies that are highly specific to these conditions. In patients with high-grade gluten-sensitive enteropathy who do not follow a gluten-free diet, these antibodies can be found in 70% to 80% of cases. Due to its high specificity, the test can potentially reduce the need for multiple small bowel biopsies, particularly benefiting pediatric evaluations including cases of failure to thrive.
Special Instructions
Not provided.
Limitations
A negative result does not exclude dermatitis herpetiformis or celiac disease diagnosis, as patients with mild gluten-sensitive enteropathy may have negative results. Interference can occur due to strong smooth muscle staining or weak EMA staining, resulting in indeterminate outcomes, necessitating further testing. Factors like specimen collection, inappropriate test selection, and interfering substances may also impact results.
New York Approved
Methodology
Immunoassay (Indirect Immunofluorescence Assay (IFA))
Biomarkers
LOINC Codes
- 46126-9 - Endomysium IgA Ser Ql IF
- 46126-9 - Endomysium IgA Ser Ql IF
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis, Gross lipemia, Gross icterus
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 30 days |