Endomysial Antibodies, IgA, Serum

Casandra

Casandra Test Code MC27212Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID EMACPT Code 86231

Endomysial Antibodies, IgA, Serum

Clinical Use

Use

The analysis of IgA-endomysial antibodies is crucial for diagnosing dermatitis herpetiformis and celiac disease. This test aids in the diagnosis by detecting circulating IgA antibodies that are highly specific to these conditions. In patients with high-grade gluten-sensitive enteropathy who do not follow a gluten-free diet, these antibodies can be found in 70% to 80% of cases. Due to its high specificity, the test can potentially reduce the need for multiple small bowel biopsies, particularly benefiting pediatric evaluations including cases of failure to thrive.

Special Instructions

This test includes a reflex to EMA Titer, S (IgA) if the result is positive or indeterminate, which is performed at an additional charge. The Celiac Disease Diagnostic Testing Algorithm and various cascade testing algorithms are available to guide test selection depending on the patient's dietary status and specific needs. When sending specimens, complete a Gastroenterology and Hepatology Test Request form if not ordering electronically. Specimens must be centrifuged and aliquoted into a plastic vial before submission.

Limitations

A negative result does not exclude dermatitis herpetiformis or celiac disease diagnosis, as patients with mild gluten-sensitive enteropathy may have negative results. Interference can occur due to strong smooth muscle staining or weak EMA staining, resulting in indeterminate outcomes, necessitating further testing. Factors like specimen collection, inappropriate test selection, and interfering substances may also impact results.

Test Details

New York Approved