Estrogens, Estrone (E1) and Estradiol (E2), Fractionated, Serum
Use
This test is useful for the simultaneous high-sensitivity determination of serum estrone and estradiol levels. It is particularly beneficial in cases requiring higher sensitivity measurement of estradiol, estrone, or both, such as the diagnosis and workup of precocious and delayed puberty, disorders of sex steroid metabolism, fracture risk assessment in postmenopausal women, and monitoring low-dose female hormone replacement therapy. It is also used for monitoring antiestrogen therapy and in settings that require moderately sensitive measurement of estradiol, such as evaluating hypogonadism and oligo-amenorrhea in females, assessing ovarian status for assisted reproduction protocols, evaluating feminization in males, and diagnosing estrogen-producing neoplasms.
Special Instructions
If not ordering electronically, complete and send General Test Request form with the specimen. Collection instructions require centrifuging and aliquoting serum into a plastic vial within 2 hours of collection. Day(s) performed are Monday through Sunday.
Limitations
Fulvestrant can cause false-high results in estradiol immunoassays but has negligible impact on measurements by mass spectrometry due to lower cross-reactivity. Estradiol levels below the premenopausal range in young females may indicate various forms of hypogonadism. Irregular menstrual periods with normal or high E2 levels may suggest polycystic ovarian syndrome or estrogen-producing tumors. Further work-up may involve additional hormone measurements and imaging. The test's accuracy may be impacted by interfering factors like improper specimen collection and handling. Estradiol analysis can inform fertility considerations and managing hormone replacement in menopausal women, making it crucial that interpretation considers all relevant medical history and concurrent testing.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 53766-2
- 2258-2
- 2243-4
Result Turnaround Time
2-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1.2 mL
Minimum Volume
0.7 mL
Container
Plastic vial
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot serum in a plastic vial.
Causes for Rejection
Serum gel/SST is not acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 28 days |
| Refrigerated | 28 days |
| Frozen | 28 days |