Flunitrazepam Confirmation, Serum
Use
This test is used for the confirmation of flunitrazepam, commonly known as Rohypnol, in serum. Flunitrazepam is a potent benzodiazepine used for short-term treatment of insomnia and as a pre-anesthetic medication. It has been associated with misuse as a 'date rape' drug due to its strong sedative effects. The test is critical in contexts where drug overdose, abuse, or compliance with prescribed usage must be assessed, as flunitrazepam is not legally marketed in the United States.
Special Instructions
Not provided.
Limitations
The test is limited by its dependence on proper specimen collection and handling. Plasma and serum must be collected in specific tubes as indicated, and refrigerants are preferred during shipping to maintain sample integrity. Hemolysis, lipemia, icterus, or other factors do not automatically cause rejection, but may impact test results. Analytical sensitivity is contingent on the instrument calibration and standard reference concentrations available for flunitrazepam.
New York Approved
Methodology
Mass Spectrometry (GC-MS)
Biomarkers
7-Aminoflunitrazepam
AnalyteFlunitrazepam
Analyte
LOINC Codes
- 73828-6 - Flunitrazepam SerPlBld-mCnc
- 73829-4 - 7Aminoflunitrazepam SerPlBld-mCnc
Result Turnaround Time
5-9 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
5 mL
Minimum Volume
2 mL
Container
Plastic vial
Collection Instructions
Draw blood in a green-top (sodium heparin) tube, spin down and send refrigerated. Plasma gel tube is not acceptable.
Storage Instructions
Refrigerated (preferred) up to 14 days, ambient for 48 hours, or frozen for 365 days.
Causes for Rejection
Improper tube type
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 14 days |
| Frozen | 365 days |