Francisella tularensis Antibody, IgM and IgG, ELISA, Serum
Use
This test is used to aid in the diagnosis of tularemia caused by Francisella tularensis. It detects IgM and IgG class antibodies in serum. Francisella tularensis is a small, intracellular, coccobacillary gram-negative bacterium, which is considered a potential agent of bioterrorism. Human infection usually occurs through inhalation of infected aerosols, ingestion of contaminated food or water, handling sick animals, or through arthropod bites. The test supports the diagnosis of current or recent tularemia by identifying IgM positivity or seroconversion to IgG in paired sera collected 2 to 3 weeks apart.
Special Instructions
Not provided.
Limitations
This test should not be used as a test of cure, as it is not quantitative. Patients may remain seropositive for months to years following the resolution of the disease. False-negative results may occur if specimens are collected too soon after symptom onset. False positives may occur due to cross-reactivity with Brucella, Yersinia, Salmonella, or Legionella infections.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 93715-1 - F. tularensis IgG+ IgM Pnl Ser
- 93716-9 - F tular IgM Ser Ql IA
- 93717-7 - F tular IgG Ser Ql IA
- 93718-5 - F tular IgG+IgM Ser-Imp
Result Turnaround Time
1-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.6 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial. Preferred container is Serum gel, but Red top is acceptable.
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus, heat inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 30 days |