Gelatin, IgE, Serum
Use
Establishing a diagnosis of an allergy to gelatin, defining the allergen responsible for eliciting signs and symptoms, identifying allergens responsible for allergic responses and/or anaphylactic episodes, confirming sensitization prior to beginning immunotherapy, and investigating the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens. Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients whose medical management does not depend on the identification of allergen specificity.
Special Instructions
For a listing of allergens available for testing, see the Allergens - Immunoglobulin E (IgE) Antibodies document.
Limitations
Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum. False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases. Test results must be interpreted in the clinical context.
Methodology
Immunoassay (FEIA)
Biomarkers
LOINC Codes
- 7332-0
- 7332-0
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens requested
Minimum Volume
For 1 allergen: 0.3 mL, For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Container
Plastic vial (Preferred: Serum gel / Acceptable: Red top)
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis and gross lipemia are acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |
