Germ Cell Tumor (GCT), Isochromosome 12p, FISH, Tissue
Use
Supporting the diagnosis of germ cell tumors when used in conjunction with an anatomic pathology consultation. Germ cell tumors comprise a heterogeneous group of neoplasms often responsive to chemotherapy, and distinguishing them from similar malignancies is critical to providing appropriate treatment. Gain of the short arm of chromosome 12, especially as an isochromosome 12p [i(12p)], serves as a chromosomal marker indicative of GCT, with diagnostic and possibly therapeutic relevance. Testing for i(12p) is recommended alongside histologic evaluation to support the diagnosis of GCT.
Special Instructions
Not provided.
Limitations
This FISH assay does not rule out other chromosome abnormalities and is best used as an adjunct to clinical and pathologic information. Non-formalin fixed specimens may have less favorable outcomes for successful results. Decalcified specimens may also be less successful, with a success rate of approximately 50%. If insufficient tissue/tumor is available for analysis, the reviewing pathologist may cancel testing.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Unknown CNV
Copy Number Region
LOINC Codes
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 62356-1 - Chrom analy result (ISCN)
- 42349-1
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 48767-8 - Annotation comment Imp
- 18771-6 - Provider signing name
- 62364-5 - Test performance info Spec
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Slides: 1 Hematoxylin and eosin stained and 2 unstained
Collection Instructions
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods are less favorable for successful FISH testing. For tissue slides, submit 4 consecutive unstained, positively charged, unbaked slides with 5 micron-thick sections of tumor tissue and 1 slide stained with hematoxylin and eosin.
Causes for Rejection
All specimens will be evaluated for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient preferred |