Grape, IgE, Serum
Use
This test is used for establishing a diagnosis of an allergy to grape by detecting IgE antibodies specific to grape allergens. It helps in defining the allergen responsible for allergic reactions, identifying allergens responsible for anaphylactic episodes, and confirming sensitization before starting immunotherapy. The test is useful in investigating the specificity of allergic reactions to insect venom, drugs, or chemical allergens. However, testing for IgE antibodies is not deemed useful in patients previously treated with immunotherapy or in cases where clinical management does not require allergen specificity.
Special Instructions
Clinicians should complete the Allergen Test Request form (T236) when not ordering electronically and send it with the specimen. For a complete list of allergens that can be tested, refer to the Allergens - Immunoglobulin E (IgE) Antibodies PDF provided. The optimal specimen collection requires the use of serum gel or red top tubes, and the serum should be centrifuged and aliquoted into a plastic vial for submission.
Limitations
Measurable levels of IgE antibodies can exist in individuals with clinically insignificant sensitivity, leading to false positives. Additionally, patients with markedly elevated serum IgE (>2500 kU/L) may experience nonspecific IgE binding to allergen solid phases. IgE antibody levels should be interpreted in the clinical context, particularly as they cannot determine residual clinical sensitivity post-immunotherapy.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 6841-1
- 6841-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens requested
Minimum Volume
For 1 allergen: 0.3 mL, for more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Serum must not be grossly hemolyzed or lipemic.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |