Heartland Virus, RNA, Molecular Detection, PCR, Serum
Use
This test aids in the diagnosis of central nervous system infection caused by the Heartland virus using serum specimens. The detection of HRTV nucleic acid in serum is a marker for acute infection caused by this virus. Important in discontinuing empiric antibiotics and providing prognostic information for patients and families, especially as HRTV disease generally presents with symptoms such as headache, fever, fatigue, anorexia, nausea, and diarrhea. It is crucial that molecular testing is performed within the first week following symptom onset for accurate diagnosis, as detection period is brief.
Special Instructions
Patients with symptoms for more than 1 week may test negative by molecular tests and might need serologic testing, available at the CDC. Upon collection, serum must be centrifuged and aliquoted into a sterile container within 2 hours. Additional serologic confirmation through CDC may be required if negative result after 7 days of symptoms.
Limitations
Negative results do not rule out infection, particularly if specimen collection occurs more than 7 days following symptom onset. Interference could arise due to improper specimen collection or handling leading to false results. This assay is not FDA-approved and should be interpreted in conjunction with clinical presentation and exposure history. Negative results may necessitate serologic testing.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 94183-1
- 94183-1
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot serum into a sterile container.
Causes for Rejection
Gross hemolysis; Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 14 days |
| Frozen | 14 days |