Heartland Virus, RNA, Molecular Detection, PCR, Spinal Fluid
Use
Aiding in the diagnosis of central nervous system infection caused by Heartland virus using spinal fluid specimens. Heartland virus (HRTV) disease is an emerging zoonosis, transmitted to humans through the bite of infected Amblyomma americanum (Lone Star) ticks. Detection of HRTV nucleic acid in cerebrospinal fluid (CSF) is a marker for central nervous system infection caused by this virus. It is important for providing prognostic information and guiding treatment decisions, as there is no targeted antiviral therapy available and treatment remains supportive.
Special Instructions
Not provided.
Limitations
Heartland virus nucleic acid may be detectable only during the first week following symptom onset; thus, molecular testing should be performed within this time frame. Negative results after this period may not rule out infection, and serologic testing may be necessary. False negatives may occur if samples are collected more than 7 days post-symptom onset. The test is qualitative and reports as either positive or negative. Results should be interpreted in conjunction with clinical presentation and exposure history.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
Heartland virus
Microorganism
LOINC Codes
- 94183-1 - HRTV RNA Spec Ql NAA+probe
- 94183-1 - HRTV RNA Spec Ql NAA+probe
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
Not provided
Container
Sterile vial
Collection Instructions
Send specimen from collection vial 2. Do not centrifuge or heat inactivate.
Causes for Rejection
Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 14 days |
| Frozen | 14 days |