Hematologic Disorders, Leukemia/Lymphoma; Flow Hold, Varies
Use
The test is critical for evaluating lymphocytoses of undetermined etiology and identifying B- and T-cell lymphoproliferative disorders involving blood and bone marrow. It aids in distinguishing acute lymphoblastic leukemia from acute myeloid leukemia (AML) and allows for the immunologic subtyping of acute leukemias. Additionally, the test is used for distinguishing reactive lymphocytes, lymphoid hyperplasia from malignant lymphoma, and between malignant lymphoma and acute leukemia. It is also useful for the phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia. Recognizing AML with minimal morphologic or cytochemical evidence of differentiation is another application, as well as recognizing monoclonal plasma cells. Notably, the test is not intended for the detection of minimal residual disease below 5% blasts.
Special Instructions
Not provided.
Limitations
Viability is assessed in all tissue specimens. Cases with less than 50% viability must be interpreted with caution and may yield false-negative results. Fine-needle aspiration and small biopsy specimens often have low cell counts and poor viability, which may be uninterpretable. The test's ability to detect minimal residual disease is limited to above 5% blasts.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- In Process
- 34574-4 - Path report.final Dx Spec
- 30954-2 - Relevant dx tests/lab data note
- 22635-7 - Path report.microscopic Spec Other Stn
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
3 mL
Container
Yellow top (ACD solution A or B); Lavender top (EDTA) or Green top (sodium heparin) acceptable
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot. Label specimen as blood.
Storage Instructions
Ambient 4 days/Refrigerated 4 days
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 days |
| Refrigerated | 4 days |