Use

The test is useful for distinguishing large deletional hereditary persistence of fetal hemoglobin (HPFH) from other conditions characterized by increased percentage of fetal hemoglobin (Hb F). It determines the distribution of Hb F within red blood cells, providing insight into conditions such as beta-thalassemia major, sickle cell disease, aplastic anemia, certain leukemias, and hemoglobinopathies associated with hemolysis. This test guides in understanding the physiologic and pathologic increases in Hb F, important in disorders like hereditary spherocytosis and alpha-thalassemia minor.