Hepatitis B Virus Genotyping
Use
The Hepatitis B Virus Genotyping test is used to identify major genotypes of the Hepatitis B Virus (HBV) and assess mutations in the viral sub-populations. Genotyping of HBV is crucial for patient management, as different genotypes may respond differently to antiviral treatments and are associated with different clinical outcomes. Moreover, the test helps in detecting resistance and surface antigen mutations in HBV, enabling personalized and effective treatment planning for patients infected with HBV.
Special Instructions
This test requires specific specimen handling and preparation to ensure accurate results. It is critical to centrifuge and aliquot 2 mL of plasma into a plastic vial and send it frozen. Make sure that the HBV Viral Load and the Viral Load Date are recorded, as the test may not be successful if the HBV Viral Load is less than log 3.0 or 1000 IU/mL of plasma.
Limitations
The test may not detect mutations in viral sub-populations that constitute less than 20% of the total virus population. Moreover, it may be unsuccessful if the HBV viral load is below log 3.0 or 1000 IU/mL. Additionally, interference in specimen collection or handling, as well as inappropriate test selection, may lead to diagnostic confusion. Heparinized specimens are not acceptable and should be avoided to prevent sample rejection.
New York Approved
Methodology
PCR-based
Biomarkers
LOINC Codes
- 32366-7
- 32366-7
Result Turnaround Time
10-21 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
0.5 mL
Container
EDTA (lavender or pink top), Plastic vial
Collection Instructions
Centrifuge and aliquot 2 mL plasma into a plastic vial. Send frozen.
Storage Instructions
Send frozen. Stable for 42 days frozen, 7 days refrigerated, 72 hours ambient.
Causes for Rejection
Heparinized specimens; other conditions causing thawing are acceptable (Cold OK; Warm OK)
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 7 days |
| Frozen | 42 days |