Hepatocyte Immunostain, Technical Component Only
Use
This test is useful for distinguishing hepatocellular carcinoma from other types of cancer. Normal liver tissue is expected to show a positive result with distinct granular cytoplasmic staining of hepatocytes. Bile ducts and nonparenchymal liver cells are expected to show a negative result.
Special Instructions
This test involves only the technical performance of the stain and does not include a pathologist's interpretation. In cases where interpretation is required, a separate pathology consultation should be ordered. The test should not be ordered with PATHC / Pathology Consultation, as these technical component stains will be canceled if ordered with PATHC. Digital images are only available when orders are placed electronically.
Limitations
The effectiveness of this test can be impacted by the age of the paraffin section, as the immunoreactivity can degrade. Environmental factors affecting the charge of glass slides may alter slide staining. Best practice suggests that paraffin sections be cut within 6 weeks of analysis. Unsuitable glass slide surfaces may also contribute to staining inconsistencies.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Storage Instructions
Ambient temperature is preferred. Refrigeration is also acceptable.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient preferred |
| Refrigerated | Refrigerated acceptable |