Herpes Simplex Virus (HSV), Molecular Detection, PCR, Blood

Casandra

Casandra Test Code MC79261Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID HSVPBCPT Code 87529

Herpes Simplex Virus (HSV), Molecular Detection, PCR, Blood

Clinical Use

Use

This qualitative molecular assay provides detection of HSV DNA in blood to aid in the rapid diagnosis of disseminated herpes simplex virus infection. It reports results as positive or negative for HSV‑1, HSV‑2, or HSV‑indeterminate, with an “invalid” result indicating inability to determine presence or absence of HSV DNA. Negative results do not rule out infection, and the test should not be used for screening asymptomatic individuals. An indeterminate result warrants repeat collection and testing by an alternate method if typing is required.

Special Instructions

Testing employs Real‑Time PCR with DNA probe hybridization. The assay is available and performed every day of the week (Monday through Sunday). For patients with localized disease, collection of lesion swabs and ordering HSVPV is recommended instead. The current performing laboratory is Mayo Clinic Laboratories – Rochester main campus. NY State is listed as available (“Yes”) for this test.

Limitations

Designed only for patients with clinical history and symptoms consistent with HSV infection; it should not be used to screen asymptomatic individuals. A negative result does not eliminate the possibility of infection. Viral DNA detection may occur in asymptomatic viral shedding. An indeterminate result occurs when HSV DNA is detected but cannot be typed; an invalid result indicates inability to detect. Improper specimen collection, handling, or interfering substances may affect accuracy.

Test Details

New York Approved