Herpes Simplex Virus (HSV), Molecular Detection, PCR, Spinal Fluid
Use
This test aids in the rapid diagnosis of herpes simplex virus (HSV)-1 and HSV-2 infections of the central nervous system (CNS). HSV infection of the CNS can result in encephalitis and meningitis, which are medical emergencies. Diagnosing these infections quickly is crucial for appropriate patient management and treatment.
Special Instructions
Specimen should be submitted from the second spinal fluid collection vial. Use sterile technique to transfer into an aliquot vial. Do not centrifuge or heat inactivate the specimen. The specimen should be processed in an environment where contamination with HSV DNA is unlikely.
Limitations
This test is not validated for sample types other than cerebrospinal fluid (CSF). Negative results do not exclude HSV-1 or HSV-2 infection and should not be the sole basis for treatment decisions. False-negatives may occur if viruses are present below the assay's sensitivity, if the virus has mutations, or if the test is conducted early in the illness. Performance has not been established for immunocompromised patients or for monitoring CNS treatment.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 92865-5
- 16952-4
- 16960-7
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
0.2 mL
Minimum Volume
0.1 mL
Container
Plastic vial
Collection Instructions
Submit from second spinal fluid collection vial. Transfer into aliquot vial using sterile technique. Do not centrifuge or heat inactivate.
Causes for Rejection
Heat-treated spinal fluid
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |