Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only
Use
Histone H3 K27M is an alteration in the H3F3A gene, encoding for histone H3.3. This alteration is characteristic of 'diffuse midline glioma, H3 K27M-mutant,' a new entity in the classification of central nervous system tumors, which carries a poor prognosis. H3 K27M-mutant diffuse midline glioma occurs most commonly in young children but, less frequently, can occur in adults. The most common locations include the brain stem, thalamus, and spinal cord. The term brain stem glioma and diffuse intrinsic pontine glioma were previously used to indicate tumors occurring in the brain stem and pons respectively.
Special Instructions
Not provided.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The positive and negative controls are verified as showing appropriate immunoreactivity. Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist. Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
H3 K27M
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides