HIV-1/HIV-2 RNA Detection, Plasma
Use
This test aids in the qualitative detection and differentiation of HIV-1 and HIV-2 infections in individuals with indeterminate or inconclusive HIV serologic test results. It is especially useful for diagnosing HIV in individuals suspected to be in the acute or early stages of HIV infection, during the so-called 'window period'. Additionally, it is used for diagnosing HIV in infants under 18 months of age who were born to HIV-infected mothers.
Special Instructions
This test involves using the Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. It should be noted that serologic testing should be completed before this test if it has not already been done.
Limitations
The test is not licensed by the FDA as a screening test for HIV-1 infection in donors of blood, human cells, tissues, or tissue products. A single 'Undetected' result may not rule out HIV infection if the plasma specimen is collected and tested too early post-exposure or if the patient is infected with a rare HIV variant. Improper storage or processing of the plasma specimen could lead to false negatives.
FDA ApprovedNew York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 96557-4
- 25835-0
- 69353-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
0.8 mL
Container
Plastic vial
Collection Instructions
Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (e.g., within 2 hours of collection). Freeze aliquoted plasma for shipment.
Storage Instructions
Ship specimen frozen on dry ice. If delayed more than 24 hours, freeze at -20 to -80 degrees C until shipment on dry ice.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 40 days |