HIV-1/HIV-2 RNA Detection Prenatal, Serum
Use
This test is useful for diagnosing HIV-1 and/or HIV-2 infection in pregnant individuals who have indeterminate or inconclusive HIV serologic test results or are suspected to be in the acute or early phase of infection. It provides qualitative detection and differentiation of HIV-1 and HIV-2, which is critical for prompt treatment and prevention of perinatal transmission.
Special Instructions
This test is specifically designed for pregnant individuals and should be used when serologic testing results are indeterminate or inconclusive. If serologic testing has not been performed, alternative tests like HVPRS or HVPPS should be ordered to ensure comprehensive evaluation. Specimens must be shipped frozen on dry ice and, if delayed, serum should be frozen at -20 to -80 degrees Celsius until shipment.
Limitations
This test is not FDA licensed as a screening test for blood donors or related products. A single 'Undetected' result may not exclude HIV infection if the specimen is collected too early post-exposure or if there is a rare variant infection. Improper specimen handling can lead to false negatives, and inhibitory substances may cause inconclusive results. Proper collection, storage, and handling are crucial.
FDA ApprovedNew York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 96557-4
- 25835-0
- 69353-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1.5 mL
Minimum Volume
0.8 mL
Container
Plastic vial
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot serum into plastic vial. Freeze aliquoted serum for shipment.
Causes for Rejection
Gross hemolysis or gross lipemia will cause rejection.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 40 days |