HIV-2 DNA/RNA Qualitative Real-Time PCR
Use
The HIV-2 DNA/RNA Qualitative Real-Time PCR test is primarily used to detect the presence of HIV-2 genetic material (DNA and RNA) in patient samples. This test is valuable for diagnosing HIV-2 infections, particularly in cases where serological tests may be inconclusive or in newly infected individuals where antibodies may not yet be present. It serves as a confirmatory test for HIV-2 infection and is essential for ruling out or confirming the presence of the virus, which is critical for monitoring and managing patients suspected of having HIV-2.
Special Instructions
The test requires whole blood collected in an EDTA tube and shipped refrigerated to ensure specimen stability. It is important to properly collect and preserve the specimen to avoid rejection. Hemolysis and heparinized blood samples are causes for rejection, adhering to the specific collection and shipping instructions is crucial.
Limitations
This test has not been cleared or approved by the FDA. It has been validated according to CLIA regulations by Quest Diagnostics and is intended for clinical use. Results must be interpreted in conjunction with clinical findings and other laboratory data. Due to the specificity of nucleic acid detection, cross-contamination or improper handling may result in erroneous results. As a qualitative assay, it does not quantify the amount of viral load present.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 25841-8
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1mL
Minimum Volume
0.5 mL
Container
EDTA Tube
Collection Instructions
Collect 1 mL (lavender-top) EDTA whole blood. Ship refrigerated.
Causes for Rejection
Hemolysis, heparinized whole blood
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 30 days |