HTLV I/II DNA, Qualitative Real-Time PCR
Use
The Human T-Lymphotropic Virus (HTLV) types I and II are associated with certain diseases, such as adult T-cell leukemia/lymphoma and HTLV-associated myelopathy. This test is used to qualitatively detect HTLV I and II DNA using PCR methodology. It assists in the diagnosis of infections caused by these viruses and helps in the management of patients, particularly for epidemiological studies and blood screening programs.
Special Instructions
This test requires whole blood collected in a lavender-top (EDTA) tube. The specimen must be sent refrigerated to ensure specimen stability and accuracy of results. It is important to follow collection and transportation guidelines precisely to avoid specimen rejection.
Limitations
The test is highly specific but may not detect low levels of viral DNA below the detection threshold of the assay. Cross-reactivity with other retroviruses is not common, but rare occurrences may affect results. False negatives may occur if the virus is present in very low quantities or is below the level of detection. Specimen quality and proper handling are critical for accurate test results. The assay has not been FDA-cleared or approved.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 44537-9
- 44542-9
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA)
Collection Instructions
Draw whole blood specimen in a lavender-top (EDTA) tube. Send refrigerated.
Causes for Rejection
Mild hemolysis is acceptable; gross hemolysis, heparin, or ACD anticoagulated specimens are unacceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |