Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Confirmation, Spinal Fluid
Use
This test is used for the confirmatory detection of human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2)-specific IgG antibodies in spinal fluid specimens that have consistently shown reactivity by initial screening tests. It helps in differentiating between HTLV-1- and HTLV-2-specific IgG antibodies present in spinal fluid. HTLV-1 infection is linked to adult T-cell leukemia or lymphoma and HTLV-1-associated myelopathy or tropical spastic paraparesis. HTLV-2 infection has been associated with conditions such as hairy-cell leukemia but lacks definitive etiological evidence. Both viruses are mainly transmitted through blood transfusion, sexual contact, contaminated needles, and from mother to fetus.
Special Instructions
For an evaluation that includes both screening and confirmation, order HTLVC / Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Screen with Confirmation, Spinal Fluid. For testing serum specimens, order HTLVL / Human T-Cell Lymphotropic Virus Types I and II (HTLV-I/-II) Antibody Confirmation, Serum. Date of specimen collection is required for processing. Gross hemolysis, and gross lipemia specimens are rejected.
Limitations
A negative line immunoassay result does not preclude the possibility of exposure to HTLV-1 or HTLV-2. Results from this confirmatory assay should always be interpreted together with the reactive screening test result on a given specimen. Confirmatory results are based on specific antibody band patterns on the assay strips, therefore repeated testing or further specimen collection might be necessary if bands do not clearly indicate HTLV IgG antibodies.
New York Approved
Methodology
Immunoassay (LIA)
Biomarkers
LOINC Codes
- 93744-1
- 93745-8
- 93743-3
- 93742-5
Result Turnaround Time
2-15 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Sterile vial
Collection Instructions
Order this confirmatory assay only on spinal fluid specimens that are consistently reactive by an antihuman T-cell lymphotropic virus 1 and 2 (HTLV-1/-2) screening immunoassay.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 30 days |