Human Transforming Growth Factor beta 1 (TGF-b1)
Use
Transforming growth factor (TGF) plays a crucial role in tissue regeneration, cell differentiation, embryonic development, and regulation of the immune system. Transforming growth factor beta is found in hematopoietic (blood-forming) tissue and initiates a signaling pathway that suppresses the early development of cancer cells. It enhances the deposition of extracellular matrix and may play a potential role in wound healing and cirrhosis formation. Many cells synthesize TGF-b and almost all of them have specific receptors for this peptide.
Special Instructions
Specimens must be processed immediately after collection as per instructions: centrifuge serum within 30 minutes of collection and follow immediate freezing steps. Specific centrifugation steps are necessary to ensure platelet-free plasma.
Limitations
Test results are not cleared or approved by the US FDA, but are used for clinical diagnosis without FDA approval. Variability in the sample preparation and handling may affect the quality and reliability of the test results. Gross hemolysis, lipemia, or particulate/microbial contamination may lead to sample rejection. Results must be interpreted in the context of clinical presentation and other laboratory findings.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
Result Turnaround Time
6-14 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.25 mL
Container
Lavender top (EDTA)
Collection Instructions
1. Draw blood in a lavender top (EDTA) tube. 2. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Centrifuge plasma again at 3000 x g for 10 minutes for complete platelet removal. 3. Aliquot plasma into a plastic vial and freeze at -20 degrees C or below. 4. Send 1 mL platelet-free plasma frozen in plastic vial.
Storage Instructions
Freeze at -20 degrees C or below.
Causes for Rejection
Gross hemolysis, gross lipemia, specimens with particulate matter or microbial contamination, specimens outside of listed stability, samples submitted without two unique identifiers and date of collection
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 14 days |