IgM Immunostain, Technical Component Only

Casandra

Casandra Test Code MC90946Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID IGMICPT Codes 88341, 88342

IgM Immunostain, Technical Component Only

Clinical Use

Use

The immunoglobulin molecules (antibodies) function as surface receptors for antigens for B lymphocytes and as secretory products of plasma cells forming the humoral arm of the immune system. IgM represents one of the immunoglobulin heavy chain types. IgM positive-B lymphocytes are normally present in the follicular mantle zones and germinal centers. Immunoreactivity is a specific marker for B lymphocytes and plasma cells. Diagnostically, staining for immunoglobulin heavy chains can aid in the diagnosis and classification of small B cell malignant lymphomas and multiple myeloma.

Special Instructions

This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Any immunohistochemistry (IHC)/in situ hybridization (ISH) stain performed as a part of the PATHC will be performed at the reviewing pathologist's discretion at an additional charge. Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Limitations

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.

Test Details

New York Approved