Insulin (Human), IgE, Serum
Use
The Insulin (Human), IgE, Serum test is useful for establishing a diagnosis of an allergy to human insulin by identifying specific IgE antibodies to insulin. It helps define the allergen responsible for eliciting allergic signs and symptoms, such as those responsible for allergic responses and anaphylactic episodes. The test also provides information useful for confirming sensitization prior to starting immunotherapy and investigating the specificity of allergic reactions to allergens such as insect venom, drugs, or chemicals.
Special Instructions
For testing, the preferred collection container is a serum gel tube, while a red top tube is acceptable. Serum should be centrifuged and aliquoted into a plastic vial as the submission container. For every allergen being tested, a specimen volume of 0.5 mL is necessary.
Limitations
Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, nor in patients where the medical management does not depend upon identification of allergen specificity. Some individuals may have measurable levels of IgE without significant clinical sensitivity, leading to possible false positives in cases of elevated serum IgE values (>2500 kU/L).
FDA ApprovedNew York Approved
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 6148-1
- 6148-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis and gross lipemia are acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |