Integrase Interactor 1 (INI1/BAF47) Immunostain, Technical Component Only
Use
Integrase interactor 1 (INI1) is a crucial component of the SWI/SNF chromatin remodeling complex, which is involved in regulating transcription by modulating DNA accessibility for proteins. Dysfunction in these complexes has been linked to various human cancers. In pediatric rhabdoid tumors, rhabdomyosarcoma, chronic myeloid leukemia, and central nervous system tumors such as medulloblastomas and choroid plexus carcinomas, alterations in the INI1/BAF47 gene lead to loss of nuclear staining, which this test identifies. In healthy tissues, nuclear expression of INI1/BAF4 is present in all cell types.
Special Instructions
Not provided.
Limitations
Age and environmental conditions of paraffin sections can impact immunoreactivity, potentially compromising results. Additionally, non-optimal glass slide charges can alter staining effectiveness, leading to inconsistent results. Proper storage and handling of slides and sections are essential to maintain test reliability.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
INI1/BAF47
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Prefer formalin-fixed, paraffin-embedded tissue block, or 2 unstained, positively charged slides (25- x 75- x 1-mm) with sections 4-microns thick per test ordered.
Storage Instructions
Attach appropriate labels to transport container.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged or inappropriate slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient preferred |