Iron, Liver Tissue
Use
This test is useful for the diagnosis of hemochromatosis using liver tissue specimens. It is designed to detect excessive iron accumulation in the liver, which is usually the first organ affected in iron-overload diseases. The test measures hepatic iron concentration and the hepatic iron index (HII), considered the 'gold standard' for diagnosis of hemochromatosis. Early diagnosis of hemochromatosis is crucial as the condition can lead to severe complications if left untreated, despite having relatively simple treatment options.
Special Instructions
Patient's date of birth is required to calculate the iron index. Paraffin blocks will be returned 7 days after analysis is complete. Form requires metal-free specimen vials and specific specimen collection instructions.
Limitations
Formalin-fixed, paraffin-embedded tissue can be used when histologic examination is requested, but fresh or frozen tissue is preferred. Paraffin blocks that have been heavily used for prior testing may be insufficient, even if they appear to contain an adequate amount of tissue. The presence of heterogeneity in liver tissue samples can lead to variability in quantitation of measured iron levels.
New York Approved
Methodology
Mass Spectrometry (ICP-MS)
Biomarkers
LOINC Codes
- 57028-3
- 57028-3
- 49061-5
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
2 mg
Minimum Volume
0.3 mg by dry weight
Container
Mayo metal-free specimen vial
Collection Instructions
Two mg of liver tissue required, typically from a 22-gauge needle biopsy at least 2 cm long. If an 18-gauge needle is used, tissue must be at least 1 cm in length.
Causes for Rejection
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 12 1/2 years |
| Refrigerated | preferred |
| Frozen | 20 years |