JC Virus, Molecular Detection, PCR, Spinal Fluid
Use
Detection of JCV DNA in cerebrospinal fluid (CSF) is useful in aiding the diagnosis of progressive multifocal leukoencephalopathy (PML) due to JC virus. This test should not be used to diagnose Creutzfeldt‑Jakob disease and is not recommended for screening asymptomatic patients.
Special Instructions
Follow the Meningitis/Encephalitis Panel Algorithm for guidance on reflex/additional testing. If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) form.
Limitations
Detection is specific for JC virus and does not cross‑react with closely related polyomaviruses such as BK virus or SV40. Detection in CSF replaces the need for brain biopsy in suspected PML cases.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 33295-7
- 33295-7
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile screw cap vial (Vial number 2 preferred; any vial number acceptable)
Collection Instructions
Do not centrifuge.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |