Legionella species, Molecular Detection, PCR, Varies
Use
This test is used for the sensitive and rapid diagnosis of pneumonia caused by Legionella species. The test is highly specific and utilizes PCR technology to detect Legionella DNA, providing quick results crucial for the early management and treatment of legionellosis. The assay detects a specific sequence within the Legionella 5S ribosomal RNA gene, which is a reliable marker for identifying the presence of the bacteria in clinical specimens.
Special Instructions
Not provided.
Limitations
The assay does not differentiate between Legionella species. False-positive results are possible if specimens are contaminated with Legionella DNA, as this organism is commonly found in the environment. The test is not suitable for use as a test of cure because bacterial nucleic acids may persist post-treatment. Moreover, some Legionella species, such as Legionella birmhinghamensis and Legionella feeleii, are not detected by this assay. Negative results do not conclusively rule out legionellosis due to possible PCR inhibition or low bacterial load below the detection limit.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
Legionella species
Microorganism
LOINC Codes
- 5020-3 - Legionella rRNA Spec Ql Probe
- 31208-2 - Specimen source
- 5020-3 - Legionella rRNA Spec Ql Probe
Result Turnaround Time
3 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
1 mL
Minimum Volume
Not provided
Container
Sterile container
Causes for Rejection
Tissue in formalin, formaldehyde, or acetone. FFPE block
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |