Legionella species, Molecular Detection, PCR, Varies

Casandra

Casandra Test Code MC64213Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID LEGRPCPT Code 87801

Legionella species, Molecular Detection, PCR, Varies

Clinical Use

Use

This test is used for the sensitive and rapid diagnosis of pneumonia caused by Legionella species. The test is highly specific and utilizes PCR technology to detect Legionella DNA, providing quick results crucial for the early management and treatment of legionellosis. The assay detects a specific sequence within the Legionella 5S ribosomal RNA gene, which is a reliable marker for identifying the presence of the bacteria in clinical specimens.

Special Instructions

This is a PCR-based test, and the high sensitivity of the amplification process requires that specimens be processed in an environment where contamination from Legionella DNA is minimized. Specimen source information must be provided, and it is important to collect specimens aseptically and ensure they are shipped promptly to maintain sample integrity. If not ordering electronically, the Microbiology Test Request Form (T244) must accompany the specimen.

Limitations

The assay does not differentiate between Legionella species. False-positive results are possible if specimens are contaminated with Legionella DNA, as this organism is commonly found in the environment. The test is not suitable for use as a test of cure because bacterial nucleic acids may persist post-treatment. Moreover, some Legionella species, such as Legionella birmhinghamensis and Legionella feeleii, are not detected by this assay. Negative results do not conclusively rule out legionellosis due to possible PCR inhibition or low bacterial load below the detection limit.

Test Details

New York Approved