Lupus Anticoagulant Evaluation with Reflex
Use
The Lupus Anticoagulant Evaluation with Reflex is used to detect lupus anticoagulants and evaluate the anticoagulant effect that can contribute to thrombosis or miscarriage. It plays a crucial role in identifying patients who may be at risk for clotting disorders and other autoimmune conditions. The reflex testing ensures that any prolonged or positive initial tests prompt further confirmatory testing to delineate risk and guide therapy.
Special Instructions
Specimens should be drawn into a light-blue top tube containing 3.2% sodium citrate. After separation of plasma, ensure that it is platelet-poor before freezing and shipping on dry ice. Incorrect collection, handling, or transport can lead to erroneous results or sample rejection.
Limitations
The test is highly dependent on proper specimen collection and handling, particularly the avoidance of platelet contamination in plasma samples. Mild hemolysis could result in rejection, while lipemic and icteric specimens are typically not affected. Reflex tests such as Hexagonal Phase Confirmation and DRVVT are only performed based on initial test results, which might extend turnaround time if confirmatory tests are required.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 75515-7
- 75514-0
- 34571-0
- 6303-2
Result Turnaround Time
2-8 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
2 mL
Container
Plastic vial
Collection Instructions
Draw blood into a 3.2% sodium citrate (light blue-top) tube. Centrifuge at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, avoiding the WBC/platelet buffy layer, and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Causes for Rejection
Mild hemolysis may result in rejection, gross hemolysis will definitely result in rejection.
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 30 days |