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Lymphocyte Proliferation to Mitogens, Blood — Mayo Clinic Lab | Casandra.ai

Casandra

Casandra Test Code MC85296Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID LPMGFCPT Code 86353

Lymphocyte Proliferation to Mitogens, Blood

Clinical Use

Use

This test is useful for assessing T-cell function in patients undergoing immunosuppressive therapy, including those with solid-organ transplants. It helps evaluate patients suspected of having impaired cellular immunity, including those with primary immunodeficiencies such as DiGeorge syndrome and severe combined immunodeficiency, as well as secondary immunodeficiencies. The test is also valuable in assessing the recovery of T-cell function following bone marrow or hematopoietic stem cell transplantation.

Special Instructions

Not provided.

Limitations

Interpretation of results may be affected by factors such as patient use of steroids or immunosuppressive agents. Specimens older than 24 hours or containing gross hemolysis or lipemia might yield unreliable results. Timing and consistency in blood collection are crucial for accurate monitoring. The test does not provide absolute correlation between in vitro T-cell proliferation and clinical immunodeficiency.

Test Details

New York Approved

Methodology

Cell-based / Cytometry (Flow Cytometry)

Biomarkers

CD19

Protein

Max Prolif of PWM as % CD19

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

CD3

Protein

Max Prolif of PHA as % CD3

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

CD45

Protein

Max Prolif of PHA as % CD45

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Order Codes

69018-0 - Lymphocyte prolif mitogen pnl Bld FC

Result Codes

33193-4 - Viable cells NFr Bld
57741-1 - LPT OKT3 Bld-nRate
69038-8 - LPT PHA max/CD45 NFr Bld FC
69037-0 - LPT PW max/CD19 NFr Bld FC
69020-6 - LPT PW/CD3 NFr Bld FC
69019-8 - LPT PW/CD45 NFr Bld FC
69052-9 - Flow cytometry specialist review
48767-8 - Annotation comment Imp

Result Turnaround Time

8-11 days

Related Documents

For more information, please review the documents below

Lab test overview

Specimen Requirements

Specimen

Whole Blood

Volume

20 mL

Minimum Volume

See Specimen Required

Container

Green top (sodium heparin)

Collection Instructions

Send whole blood specimen in the original tube. Do not aliquot.

Patient Preparation

For serial monitoring, it is recommended that specimen collection be performed at the same time of day.

Causes for Rejection

Gross hemolysis, Gross lipemia

Stability Requirements

Temperature	Period
Room Temperature	48 hours