MayoComplete Endometrial Carcinoma Panel, Next-Generation Sequencing, Tumor
Use
Primarily for determining if patients will respond to targeted therapy. Assessment of microsatellite instability for immunotherapy decisions. Molecular-based classification of endometrial carcinoma. This panel includes a curated list of 19 genes important for the clinical management of patients with endometrial cancer based on current guidelines and WHO recommendations. Microsatellite instability and mismatch repair status are evaluated, which are clinically actionable for determining immunotherapy efficacy in solid tumors.
Special Instructions
A pathology report containing patient name, block number, tissue collection date, and source of tissue must accompany specimen. This assay requires at least 20% tumor nuclei. Submit tissue block with adequate tumor tissue or slides with either 5000 nucleated cells preferred or at least 3000 nucleated cells on cytology slides. Glass coverslips are preferred over plastic to avoid longer turnaround times.
Limitations
This test cannot differentiate between somatic and germline mutations. Additional testing may be required to assess the clinical significance of reported variants. The assay detects point mutations and small delins, but not large-scale genomic copy number variants, copy neutral LOH, or promoter methylation. Variants in PMS2 exons 11-15 cannot be distinguished from its pseudogene PMS2CL. The analytical sensitivity for sequence reportable alterations is 5% mutant allele frequency with a minimum coverage of 500X. Results should be interpreted in the context of clinical and other laboratory data.
New York Approved
Methodology
NGS (Targeted)
Biomarkers
LOINC Codes
- 105590-4
- 82939-0
- 69047-9
- 48767-8
- 31208-2
- 80398-1
- 85069-3
- 62364-5
- 18771-6
Result Turnaround Time
12-20 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed paraffin-embedded tissue block or slides
Collection Instructions
Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue. Alternatively, submit 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides cut at 5-micron thick sections of the tumor tissue.
Storage Instructions
Ambient (preferred) or refrigerated storage acceptable.