Mesothelioma, CDKN2A FISH, Tissue
Use
Supporting the diagnosis of mesothelioma when used in conjunction with an anatomic pathology consultation. The histologic distinction of malignant mesothelioma from benign mesothelial proliferations can be challenging. Loss of both copies of CDKN2A has been described as a recurrent abnormality in 59% to 80% of pleural malignant mesotheliomas depending on histologic features. Homozygous deletion of CDKN2A is less common in peritoneal mesothelioma, reported to occur in 25% to 35% of cases. The detection of homozygous deletion of CDKN2A by fluorescence in situ hybridization has been suggested as a useful adjunct to histologic examination in the differentiation of malignant mesothelioma from other processes.
Special Instructions
Not provided.
Limitations
This test cannot detect all types of CDKN2A deletions. It is not approved by the FDA and should be used as an adjunct to existing clinical and pathologic information. Fluorescence in situ hybridization cannot identify all CDKN2A deletions. Fixatives other than formalin may not be successful for FISH assays. Non-formalin fixed specimens will not be rejected, but success rates are approximately 50% for decalcified tissues. Failure to detect FISH signals post-hybridization will lead to a lack of results being reported.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
No genes
Gene
LOINC Codes
- 21614-3 - CDKN2A Del Bld/T Ql
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 62356-1 - Chrom analy result (ISCN)
- 42349-1
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 48767-8 - Annotation comment Imp
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
formalin-fixed, paraffin-embedded (FFPE) tumor tissue block or slides
Collection Instructions
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Submit 4 consecutive unstained, positively charged, unbaked slides with 5 micron-thick sections of the tumor tissue and 1 slide stained with hematoxylin and eosin.
Causes for Rejection
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |