MET (7q31), FISH, Tissue
Use
Providing prognostic information and guiding treatment, primarily for patients with lung, gastric, and colorectal tumors, as well as other tumor types. MET amplification is associated with disease progression and has been reported in several types of cancer, including colorectal, gastric, and gastroesophageal adenocarcinoma. It is a mechanism for acquired resistance to EGFR-TKI therapies.
Special Instructions
Not provided.
Limitations
This test is not FDA approved and serves as an adjunct to existing clinical and pathologic information. Fresh tissue is not acceptable, and fixatives other than formalin may not be successful. The FISH assay does not rule out other chromosomal abnormalities, and paraffin-embedded tissues that have been decalcified have a lower success rate for FISH analysis. If no cells are present for analysis or sufficient tumor tissue is unavailable, testing may be canceled.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
No genes
Gene
LOINC Codes
- 90926-7 - MET amp Bld/T FISH
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 62356-1 - Chrom analy result (ISCN)
- 42349-1
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 48767-8 - Annotation comment Imp
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Submit a formalin-fixed, paraffin-embedded tumor tissue block. Provide fixation method used. Paraffin-embedded specimens can be from any anatomic location. Decalcified paraffin-embedded specimens will have testing attempted, but success rate is approximately 50%.
Causes for Rejection
Fresh tissue
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |