Metallothionein Immunostain, Technical Component Only

Casandra

Casandra Test Code MC59573Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID MTCPT Codes 88341, 88342

Metallothionein Immunostain, Technical Component Only

Clinical Use

Use

This test is intended to identify the presence of metallothionein (MT), which is useful to support a diagnosis of Wilson disease (WD). MT plays a role in binding the accumulated copper in WD disorder. MT can detect even early-stage cases of WD before the development of cirrhosis. The MT assay has higher sensitivity and specificity compared to the use of Leipzig criteria and rhodanine. MT expression by IHC is a cost-effective and practical approach compared to quantitative liver copper assays.

Special Instructions

This test includes only the technical performance of the stain and does not provide pathologist interpretation. If pathologist interpretation is required, order PATHC / Pathology Consultation. The test is not to be ordered with PATHC; technical component stains will be canceled if ordered with PATHC.

Limitations

The test does not include pathologist interpretation; it's limited to technical performance of the stain. Interpretation should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist. Age of a cut paraffin section and glass slide environmental conditions may impact immunoreactivity. Best practices include cutting paraffin sections within 6 weeks and storing charged slides in optimal conditions to avoid inconsistent staining.

Test Details

FDA Approved

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

Metallothionein

Protein

Immunoreactivity • Expression • Categorical (e.g., Positive / Negative / Indeterminate)

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Immunostain Technical Only Envelope

Collection Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label to the transport container. Use formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (4 microns thick) per test ordered.