Microsporidia Stain, Varies
Use
Diagnosis of extra‑intestinal microsporidiosis involving the lung, skin, and other organs, particularly in immunocompromised hosts. Diagnosis of ocular microsporidiosis. Microsporidia are highly specialized fungi causing a wide variety of clinical syndromes in humans, particularly among immunocompromised patients. The stain is reserved for non‑stool/non‑urine specimens and serves for microscopic identification of characteristic small spores (1–4 µm), though microscopic identification is challenging. This method complements molecular detection methods such as species‑specific PCR. A positive result suggests active or recent infection; negative result does not always rule out the disease.
Special Instructions
Method Name: Trichrome‑Blue Stain (Ryan Modification). Use per Parasitic Investigation of Stool Specimens Algorithm. Reporting Name: Microsporidia Stain. Aliases include Encephalitozoon, Enterocytozoon, Nosema, Pleistophora. NY State–orderable: Yes. Performing Laboratory: Mayo Clinic Laboratories – Rochester Main Campus. CLIA Number: 24D0404292.
Limitations
Microscopic identification can be challenging due to small spore size and resemblance to yeast. A negative result may not rule out infection due to intermittent shedding or low organism burden. Cautions include difficulty differentiating organisms from debris in feces.
New York Approved
Methodology
Immunoassay (Lateral Flow)
Biomarkers
LOINC Codes
- 10857-1
- 10857-1
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
0.5 mL
Minimum Volume
Not provided
Container
Sterile container
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | <10 days |
| Refrigerated | <3 days |
| Frozen | <10 days |