Midazolam (Versed), serum
Use
Midazolam (Versed) testing is used to monitor serum levels of the medication in patients undergoing treatment. Midazolam is a benzodiazepine medication primarily used for anesthesia, procedural sedation, trouble sleeping, and severe agitation. Monitoring is important in cases where patients might be at risk of side effects due to incorrect dosage or interactions with other drugs. This test ensures that the therapeutic levels are achieved without reaching toxic levels, thereby optimizing patient safety and treatment efficacy.
Special Instructions
Specimen collection should be careful to avoid gel separation tubes which are not acceptable for this test. Blood samples should be drawn in the appropriate tubes and spun down immediately after collection to ensure sample stability and accuracy of results.
Limitations
It’s important to note that while this test measures the concentration of midazolam in the serum, it does not measure the drug's active metabolites which could also contribute to the therapeutic or toxic effects. Also, variations in plasma protein binding can influence total serum drug levels. The test does not differentiate between the effects of different benzodiazepines if patients are consuming more than one.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 59711-2
- 59711-2
Result Turnaround Time
5-9 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
0.3 mL
Container
Plastic vial
Collection Instructions
Draw blood in a green-top (sodium heparin) tube(s), plasma gel tube is not acceptable. Spin down and send sodium heparin plasma refrigerated in a plastic vial.
Storage Instructions
Refrigerated (preferred) for up to 14 days. Stable at ambient temperature for 72 hours and frozen for 180 days.
Causes for Rejection
NA
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 14 days |
| Frozen | 180 days |