Monoclonal Protein Quantitation, Random, Urine
Use
This test is useful for identifying monoclonal gammopathies by analyzing proteins in a random urine specimen. It characterizes urine protein fractions—including albumin, alpha‑1, alpha‑2, beta and gamma globulins—and quantifies monoclonal proteins (‘M spike’). It aids in diagnosing conditions such as multiple myeloma, primary systemic amyloidosis, light‑chain deposition disease, MGUS, and idiopathic Bence Jones proteinuria. Light chains or heavy‑chain fragments in the urine may indicate renal injury (e.g., Fanconi syndrome), deposition disease, or renal insufficiency.([mayocliniclabs.com](https://www.mayocliniclabs.com/test-catalog/Overview/616909?utm_source=openai))
Special Instructions
Ordering the renal diagnostics test requires if not ordered electronically, completion and submission of the Renal Diagnostics Test Request form (T830). The test profile includes Protein Electrophoresis, Random, U (RPEU) and Protein/Creatinine Ratio, Random, U (RPTU2), and may reflex to M‑protein Mass‑Fix, Random, U (MPTRU) if a discrete electrophoresis band is detected, at additional charge. Algorithm guidance includes workflows for amyloidosis and multiple myeloma.([mayocliniclabs.com](https://www.mayocliniclabs.com/test-catalog/Overview/616909?utm_source=openai))
Limitations
Random urine is sufficient for detection but 24‑hour urine is preferred for quantitation and monitoring. Urine specimens may be rejected if unsuitable; all are evaluated for suitability. Hemolysis can cause spurious electrophoretic bands; radiographic agents and penicillin may interfere. Monoclonal gammopathies are rarely seen in patients younger than 30. A normal protein electrophoretic pattern does not exclude monoclonal gammopathy—M‑protein isotyping may be needed.([mayocliniclabs.com](https://www.mayocliniclabs.com/test-catalog/Overview/616909?utm_source=openai))
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
LOINC Codes
- 101668-2
- 44293-9
- 40661-1
- 49299-1
- 6942-7
- 9734-5
- 38190-5
- 9744-4
- 9745-1
- 2161-8
- 2890-2
- 2888-6
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
50 mL
Minimum Volume
30 mL
Container
Plastic, 60‑mL urine bottle
Collection Instructions
Collect random urine specimen; aliquot between 30 mL and 50 mL into a plastic, 60‑mL urine bottle.
Storage Instructions
Refrigerate specimen after collection and send refrigerated.
Causes for Rejection
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 14 days |
| Frozen | 5 days |