Monocyte Repartition by CD14/CD16, Blood
Use
This test aids in the diagnosis and monitoring of chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome/myeloproliferative neoplasm overlap syndrome. It is characterized by peripheral blood monocytosis with an absolute monocyte count ≥1.0 x 10⁹/L and ≥10% of the total white blood cell count persisting for 3 months or longer. Monocytes are classified into classical (MO1), intermediate (MO2), and non-classical (MO3) subsets. A characteristic increase in classical monocytes (≥94%) has been demonstrated in CMML patients, distinguishing them from other causes of monocytosis with over 90% sensitivity and specificity. This panel analyzes the repartition of monocytes to provide a semiquantitative value for the MO1 compartment, aiding in differential diagnosis and monitoring of CMML.
Special Instructions
Specimens must be refrigerated and received within 3 days of collection. When ordering, send whole blood specimen in the original tube without aliquoting.
Limitations
An expanded classical monocytes (MO1) fraction, though seen in CMML, is not diagnostic as it can also occur in other myeloid neoplasms or reactive conditions. A normal MO1 fraction doesn't exclude CMML, as it might still be present in cases with autoimmune states or post-therapy. Correlation with bone marrow and clinical history is essential for definitive diagnosis.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 101146-9
- 101147-7
- 101148-5
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
1 mL
Container
Yellow top (ACD solution A or B) preferred; Lavender top (EDTA) acceptable
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 72 hours |