Mpox Virus DNA, Qualitative Real-Time PCR
Use
The Mpox Virus DNA, Qualitative Real-Time PCR test is crucial for detecting the DNA of the Mpox virus (also known as monkeypox). It aids in diagnosing infections caused by different clades of the virus including the West African Clade. Real-Time PCR testing is essential for accurate and timely diagnosis of contagious viral infections, ensuring appropriate management and control measures can be implemented. This test is particularly significant due to the need for quick identification in outbreak scenarios, where rapid diagnosis facilitates timely treatment and public health responses.
Special Instructions
This test requires the collection of a lesion swab using proper viral transport media. It is important to vigorously swab the lesion and store the specimen in suitable media before shipping it frozen to the laboratory. Appropriate clinical information, such as patient race, ethnicity, specimen source, and anatomical location of collection, should accompany the specimen. Each sample must be stored in its own sealed bag and individually submitted.
Limitations
The test is designed specifically for the detection of Mpox virus DNA and may not identify other pathogens. It relies on the quality and quantity of the specimen; insufficient or poorly handled samples can result in false negatives. Additionally, incorrect collection methods, such as using dry swabs or specimens not properly stored in viral transport media, can lead to rejection and inaccurate results. It's important to follow standardized collection and transport procedures to ensure the reliability of the test outcomes.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 100434-0
- 100888-7
- 31208-2
- 39111-0
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
1 swab
Minimum Volume
0.5 mL
Container
Viral Culture Media (VCM), Viral Transport Media (VTM), Universal Transport Media (UTM), or equivalent
Collection Instructions
Swab the pustule/lesion vigorously and place the swab in 3 mL Viral Culture Media (VCM), Viral Transport Media (VTM), Universal Transport Media (UTM), or equivalent. Include Patient Race, Ethnicity, Specimen type (source), and Anatomic Location specimen was collected.
Storage Instructions
Ship frozen.
Causes for Rejection
Calcium Alginate swabs, Cotton swabs, Wooden shaft swabs, Dry swabs (e.g., not submitted in VCM or equivalent)
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 30 days |