MSH2 Immunostain, Technical Component Only
Use
This test is useful for identifying patients at high risk for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and hereditary endometrial carcinoma by evaluating tumor tissue in an immunopanel that includes MSH2 and other mismatch repair markers. Lynch syndrome is associated with germline variants in mismatch repair genes like MLH1 and MSH2. This test helps pinpoint the defective mismatch repair gene in tumors, offering a strategy for risk stratification and indicating the need for genetic testing.
Special Instructions
This test covers only the technical performance of the stain without pathologist interpretation. Technical component stains should not be ordered with a pathology consultation as they will be canceled. Appropriate labels for shipping should be used, and digital images are accessible through electronic orders.
Limitations
The test does not provide a pathologist interpretation, focusing only on technical staining. Staining quality can be affected by the age of paraffin sections and glass slide conditions, which may influence immunoreactivity. Moreover, the absence of MLH1 or PMS2 protein in a tumor could be linked to somatic changes rather than germline variants, and genetic testing may be required for a clear distinction. Besides, MSI-H phenotypes in some tumors might be due to somatic changes.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |