MSH6 Immunostain, Technical Component Only
Use
This test is useful for identifying patients at high risk for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and hereditary endometrial carcinoma in an immunopanel including MSH6 and other mismatch repair markers. It involves evaluating tumor tissue for mismatch repair deficiencies using immunohistochemistry (IHC) to identify patients who might benefit from further genetic testing.
Special Instructions
Not provided.
Limitations
The test does not provide pathologist interpretation and is intended only for technical performance of the stain. Age of paraffin sections and improper slide handling can influence immunoreactivity. Stability varies among antigens. Best practice is for paraffin sections to be cut within 6 weeks and avoid high humidity and heat exposure for positively charged slides. This limit test also does not differentiate between germline and somatic mutations without genetic testing. MSI-H phenotypes and loss of protein expression in various sporadic cancers do not quickly distinguish an etiology.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
MSH6
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides will be rejected.