Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Varies
Use
The test is used for the rapid detection of Mycobacterium tuberculosis complex DNA, which is vital due to the serious nature of tuberculosis and its potential to develop resistance against multiple antibiotics if treatment is not started promptly. It directly amplifies DNA from patient specimens without the need for culture growth, providing quick results which are critical in a public health setting.
Special Instructions
This test must be ordered alongside a mycobacterial culture, as PCR alone lacks the sensitivity of culture methods. Fresh, undigested specimens are preferred, and specifics on acceptable and preferred specimen types must be adhered to ensure accurate results.
Limitations
The test does not evaluate rifampin resistance and cannot substitute tests for latent tuberculosis detection. It also cannot measure bacteriologic cure or monitor therapeutic response. A negative PCR result does not rule out the presence of M. tuberculosis due to possible low organism levels below the detection threshold. Additionally, testing sensitivity can vary based on specimen type, notably lower in smear-negative cases.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 38379-4
- 31208-2
- 38379-4
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Body Fluid
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container
Collection Instructions
Submit fresh, non-digested body fluids only.
Causes for Rejection
Blood, specimens in anaerobe vial or viral transport medium, swabs, tissues in formalin.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |