Mycoplasma (Mycoplasmoides) pneumoniae Macrolide (Azithromycin) Resistance Prediction, Molecular Detection, PCR, Varies
Use
This test is used for predicting macrolide susceptibility in Mycoplasma (Mycoplasmoides) pneumoniae. Macrolide resistance in M. pneumoniae is a clinical concern due to its reported high rates in some geographic areas. The test assesses for common mutations in the 23S rRNA gene associated with high-level macrolide resistance, particularly focusing on mutations at positions 2063 and 2064. The resistance prediction aids in determining the appropriate antibiotic therapy, especially in cases of respiratory tract infections where macrolides are considered for treatment.
Special Instructions
The test is intended as a reflex for previously identified M. pneumoniae positive samples using a nucleic acid amplification test. It should be ordered only on specimens that have tested positive for M. pneumoniae. Proper collection and handling to avoid contamination are emphasized, and electronic ordering is preferred to streamline workflow.
Limitations
This assay is specific for detecting the two most common mutations associated with macrolide resistance in the 23S rRNA gene of M. pneumoniae. It does not assess other resistance mechanisms or resistance to non-macrolide antibiotics. Improper specimen collection or handling, inappropriate test selection, or interfering substances may affect test results. Results should be used alongside other clinical data and tests to inform treatment decisions.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 6483-2
- 6483-2
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Oropharyngeal Swab
Volume
Swab
Minimum Volume
Not provided
Container
Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Collection Instructions
Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells. Place swab back into swab cylinder.
Storage Instructions
Transport refrigerated (preferred) or frozen.
Causes for Rejection
Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal; Port-a-Cul tube; Anaerobic fluid vials; Dry swab (no pledget or sponge); Respiratory fluid specimens placed in viral transport medium (VTM) or placed on a swab and then into VTM (M4-RT, M4, or M5); Body fluid specimens placed in viral transport medium (VTM) or placed on a swab and then in VTM (M4-RT, M4, or M5)
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |