Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies
Use
This test is used for diagnosing infections due to Mycoplasma (Mycoplasmoides) pneumoniae and assessing macrolide susceptibility. Mycoplasma pneumoniae is a bacterium that causes upper respiratory infections, pharyngitis, and tracheobronchitis, particularly in children. This test uses real-time polymerase chain reaction (PCR) technology to rapidly and sensitively detect M. pneumoniae DNA from clinical specimens. The test is especially useful due to the limitations of culture-based and serologic assays in diagnosing M. pneumoniae infections. Additionally, macrolide resistance in M. pneumoniae is increasingly common, and this test can assess for common mutations associated with this resistance.
Special Instructions
Not provided.
Limitations
A positive result indicates the presence of Mycoplasma (Mycoplasmoides) pneumoniae, but a negative result does not rule out infection due to the presence of inhibitors within the specimen matrix or the presence of target DNA below the limit of detection. This assay should only be used for respiratory tract specimens and selected body fluids like pleural/chest fluid, pericardial fluid, and cerebrospinal fluid. The limit of detection is less than 5 target copies/mcL for all validated specimen types.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
Macrolide resistance mutations
MicroorganismMycoplasma pneumoniae
Microorganism
LOINC Codes
- 29257-3 - M pneumo DNA Spec Ql NAA+probe
- 29257-3 - M pneumo DNA Spec Ql NAA+probe
- 31208-2 - Specimen source
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
Not provided
Minimum Volume
Not provided
Container
Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Collection Instructions
Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
Causes for Rejection
Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, Anaerobic fluid vials, Dry swab (no pledget or sponge), Respiratory fluid specimens placed in VTM or placed on a swab and then into VTM (M4-RT, M4, or M5), Body fluid specimens placed in VTM or placed on a swab and then in VTM (M4-RT, M4, or M5)
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |