MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies
Use
This test is useful for establishing the diagnosis of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia and helping to distinguish it from other subtypes of low-grade B-cell lymphoma. The MYD88 L265P mutation is present in 67% to 100% of patients with lymphoplasmacytic lymphoma, often associated with clinical manifestations of Waldenstrom macroglobulinemia.
Special Instructions
Whole blood or bone marrow specimens must arrive within 10 days of collection. If the quality of the biopsy specimen is poor, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate. DNA must be extracted in a CLIA-certified laboratory or equivalent, and must be extracted from a listed acceptable specimen type. If testing fails, one repeat will be attempted, and if unsuccessful, a charge will be applied.
Limitations
The MYD88 test is a targeted assay and will not detect any mutation at codon 265 that does not result in the L>P amino acid change. Additional MYD88 alterations, including insertion or deletion events, will not be detected. The analytical sensitivity of the assay may be affected by tumor burden, fixation of paraffin-embedded specimens, or PCR interferences.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 82140-5
- 31208-2
- 82140-5
- 77202-0
Result Turnaround Time
5-8 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA)
Collection Instructions
Invert several times to mix bone marrow. Send in original tube. Label as bone marrow.
Storage Instructions
Ambient (preferred) 10 days/Refrigerated 10 days
Causes for Rejection
Decalcified bone marrow core biopsies are not acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 10 days |
| Refrigerated | 10 days |
| Frozen | Frozen |