Myeloproliferative Neoplasm, CALR with Reflex to MPL, Varies
Use
This test aids in the distinction between a reactive cytosis and a myeloproliferative neoplasm when JAK2V617F testing result is negative. It reflexively evaluates for variants in the CALR and MPL genes commonly associated with BCR::ABL1-negative myeloproliferative neoplasms. The testing sequence is based on the reported frequency of gene variants in this disease group. It is usually ordered when a JAK2 V617F result is known to be negative.
Special Instructions
Pertinent clinical history, clinical or morphologic suspicion, and date of collection are required. Specimen must arrive within 7 days of collection. If not ordering electronically, complete and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Limitations
A positive result is not specific for a particular subtype of myeloproliferative neoplasm and clinicopathologic correlation is necessary in all cases. A negative result does not exclude the presence of a myeloproliferative neoplasm or other neoplastic process.
New York Approved
Methodology
PCR-based (Fragment Analysis)
Biomarkers
LOINC Codes
- 82939-0
- 31208-2
- 50398-7
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA) or yellow top (ACD-B)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot. Label specimen as blood.
Causes for Rejection
Gross hemolysis, paraffin-embedded bone marrow aspirate clot or biopsy blocks, slides, paraffin shavings, moderately to severely clotted.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | preferred |