NAbFeron (IFNB-1) Neutralizing Antibody Test
Use
The NAbFeron (IFNB-1) Neutralizing Antibody Test is used to detect the presence of neutralizing antibodies to interferon beta, which may be associated with reduced clinical effectiveness of interferon beta therapy in patients. Neutralizing antibodies, especially when present in persistently high titers, can impact therapeutic outcomes in conditions treated with interferon beta, such as multiple sclerosis. The test results should be interpreted in the context of the patient's clinical presentation and medical history.
Special Instructions
Specimen should be collected either before treatment with interferon or more than 24 hours following the most recent dose. Patients should not be on steroid therapy for at least two weeks prior to testing. The specimen must be centrifuged and aliquoted into a plastic vial within 48 hours of collection and sent refrigerated.
Limitations
The presence of neutralizing antibodies may affect the efficacy of interferon beta therapy. False positive or negative results can occur, and all results should be interpreted considering clinical findings, patient history, and other laboratory data. The test is not FDA cleared or approved, and results should thus be used with caution, recognizing the potential for diagnostic confusion due to improper specimen handling or interfering substances.
New York Approved
Methodology
Cell-based / Cytometry
Biomarkers
Result Turnaround Time
14-25 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Within 48 hours of collection, centrifuge and aliquot 2 mL of serum into a plastic vial. Send refrigerated.
Patient Preparation
Specimen should be collected either before treatment with interferon or more than 24 hours following the most recent dose. Patient should not be on steroid therapy for at least two weeks prior to testing.
Storage Instructions
Refrigerated (preferred)
Causes for Rejection
All specimens will be evaluated by the processing and performing laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 28 days |
| Frozen | 180 days |