Phenosense HIV Drug Resistance Replication Capacity
Use
This test determines viral phenotype resistance to three classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), non-nucleoside reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI). It also assesses viral replication capacity, which is valuable in managing and monitoring therapy for HIV-1 patients with documented infection and significant viral loads.
Special Instructions
This test should be used for patients with documented HIV-1 infection and viral loads of 500 copies/mL or more. For best results, confirm viral loads within two weeks prior to submission for testing at Monogram. Separate specimens should be submitted for multiple tests to avoid delays in turnaround time. Additionally, patient samples submitted within 30 days apart will be considered duplicates and canceled.
Limitations
The test is intended for use only for patients with viral loads greater than or equal to 500 copies/mL. It may not be suitable or accurate for patients with significantly lower viral loads or for those who have recently undergone treatment modification. The assay's performance may vary depending on the subtype and mutations of the virus. Specimens that are not centrifuged, have insufficient volume, are heparinized, or have undergone repeated freeze/thaw cycles may be rejected.
New York Approved
Methodology
Culture-based
Biomarkers
LOINC Codes
- 49665-3
Result Turnaround Time
18-26 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
1 mL
Container
Lavender-top (EDTA) or plasma preparation tube (PPT)
Collection Instructions
Draw blood into two 5-mL PPT (pearl top) or EDTA (lavender top) tubes. Centrifuge specimen within 6 hours of collection. Transfer plasma to one or more polypropylene screw-capped tube(s) and freeze immediately. Send 3 mL plasma in a screw-cap vial frozen.
Patient Preparation
Patients should have their viral loads confirmed within two weeks prior to submission for testing at Monogram.
Causes for Rejection
PPT not centrifuged; insufficient volume; heparinized plasma; non-frozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in 'pop-top' or 'snap-cap' tube
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 14 days |